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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI

According to CCK-8 experiments, DHT treatment led to a marked reduction in KGN cell viability, but PLB treatment significantly enhanced it (Fig

Second, it has an additional 13 amino acids at its N-terminus, bringing the total to 83 amino acids compared to the 70 of standard IGF-1

For effective weight loss, the target dose is 2.4 mg weekly , reached after the initial 16-week titration period